The high quality of Milestone frozen sections can only be obtained by freezing the specimens with PrestoCHILL and by processing the slides with FineFIX (patent n. EP 1 455 174 B1) before staining. FineFIX is an ethanol-based patented fixation and processing reagent. Its formulation with low-toxicity additives overcomes the drawbacks commonly associated with the use of pure ethanol or ethanol-based fixatives, e.g. significant tissue shrinkage, vacuolization and pyknotic nuclei.
The FineFIX Module consists of a heated metal vessel in which the FineFIX solution is kept at 40°C. Frozen section slides are immersed in the solution for 15-60 seconds in order to simultaneously carry out the specimen’s fixation, dehydration and lipids extraction. After this pre-step, the specimens are stained according to the user’s standard protocols.