Product Details
abberior CAGE dyes represent a sophisticated family of fluorophores specifically engineered for advanced imaging techniques such as PALM, STORM, and GSD microscopy. These dyes exhibit a unique property: they remain non-fluorescent until activated by UV light. Upon exposure, they are transformed into intensely bright organic probes, delivering exceptional fluorescence with remarkable precision for localization. This uncaging mechanism makes them invaluable for applications requiring the highest levels of accuracy and brightness in single-molecule and super-resolution microscopy, significantly enhancing imaging outcomes and data reliability.
abberior CAGE 500 : It is an innovative caged dye that starts as a colourless and nonfluorescent molecule. Upon exposure to ultraviolet (UV) light, it undergoes a swift photoactivation process, known as uncaging, to release a highly fluorescent dye. This activated dye exhibits a fluorescence peak at 525 nm, making it ideal for various advanced imaging techniques.
Specifically crafted for single-molecule photoswitching microscopy, abberior CAGE 500 delivers exceptional performance in methods such as PALM (Photoactivated Localization Microscopy), STORM (Stochastic Optical Reconstruction Microscopy), and GSDIM (Ground State Depletion with Individual Molecule Return). Additionally, once uncaged, it seamlessly integrates into STED (Stimulated Emission Depletion) microscopy, enhancing imaging resolution and detail.
Beyond imaging, abberior CAGE 500 serves as a powerful tool for tracking molecular dynamics. After photoactivation, researchers can observe and analyze real-time processes such as molecular diffusion, directional flows, and velocity measurements. These tracking experiments capture the behaviour and movement of molecules over time, providing crucial insights into complex biological systems.
abberior CAGE 552 : abberior CAGE 552 is a specialized caged dye that starts off as a nonfluorescent and colourless compound. Upon exposure to light in the 360 - 440 nm range, it transforms into a vibrant red, highly fluorescent dye with an absorption peak at 552 nm and emission around 575 nm.
This dye is ideal for single-molecule photoswitching microscopy techniques, including PALM, STORM, and GSDIM. Once activated, abberior CAGE 552 is also effective for STED microscopy, with a recommended depletion wavelength between 650 - 775 nm. Its uncaged form is known for exceptional brightness and impressive photostability, making it a reliable choice for high-precision imaging.
As the smallest and lightest caged rhodamine dye available, abberior CAGE 552 stands out in its category. Post-photoactivation, it is particularly useful for tracking molecular dynamics, allowing researchers to study diffusion, flow patterns, and velocity by monitoring the fluorescence signal over time.
abberior CAGE 590 : abberior CAGE 590 is a unique, non-fluorescent caged dye that becomes brightly fluorescent upon exposure to UV light. This rapid uncaging reaction produces a vivid orange-red dye, which is most efficiently excited within the 560 to 600 nm wavelength range.
Specifically developed for single-molecule photoswitching microscopy techniques like PALM, STORM, and GSDIM, abberior CAGE 590 also delivers outstanding performance in STED microscopy in its uncaged state. Post-photoactivation, is ideal for tracking molecular dynamics in confocal and epifluorescence microscopy, allowing researchers to study diffusion, flow patterns, and velocity in real-time. This makes it highly suitable for experiments where fluorescence is monitored over time to gain insights into molecular movement and behaviour.
abberior CAGE 635 : The abberior CAGE 635 is a specialized caged dye that starts as colourless and non-fluorescent. When exposed to UV light, it rapidly undergoes an uncaging reaction, transforming into a highly fluorescent red dye. It is optimally excited in the 620 to 645 nm wavelength range. abberior CAGE 635 is ideal for advanced single-molecule photoswitching microscopy techniques, including PALM, STORM, and GSDIM. Once uncaged, it also demonstrates excellent performance in STED microscopy. Additionally, after activation, the dye can be used in confocal and epifluorescence microscopy for tracking molecular dynamics such as diffusion, flow direction, and velocity, making it suitable for experiments that monitor fluorescence over time after the dye is released.