Ablate!™ laser ablation system is an attenuatable 532nm pulse laser (>60µJ pulses at 200Hz) that inflicts localized damage to a diffraction-limited spot in intracellular structures for precise laser ablation and laser wounding. Ablate! has automated intensity control and is available in fixed-point and scanning versions. Direct coupling to the microscope allows for optimal power delivery and available notch dichroic filter sets enable simultaneous widefield or confocal imaging. Ablate! includes the SlideBook Photomanipulation module which allows the user to define a region or regions of interest as a single diffraction limited spot or as user defined shapes.