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Real -Time PCR Kits For Breast Cancer

ENTROGEN Real -Time PCR Kits For Breast Cancer Kit in India in India

Real -Time PCR Kits For Breast Cancer
EntroGen’s mutation analysis kit requires a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes.
This test includes reagents required for the PCR amplification/detection, as well as validated reaction controls. Columns and reagents for DNA isolation are not included.
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Features & Benefits

1. Below 1% Limit of Detection (LOD)

2. Highly sensitive and specific Works with FFPE, fresh frozen and FNA samples

3. Simple setup and interpretation Includes internal control

4. Available as RUO.

Product Details

Entrogen's Real Time PCR Kits for Breast Cancer are available as following three categories :


Product Name

Catalog Number


AKT E17K Mutation Analysis Kit


For Real-Time PCR

PIK3CA Mutation Analysis Kit


For Real-Time PCR

BRCA Complete


For Illumina Platforms

1. AKT1 is a member of serine-threonine kinases that play a key role in various cellular processes including growth, proliferation, survival and angiogenesis. AKT1 is a downstream mediator of PI3 kinase. A somatic mutation that substitutes glutamic acid (E) with lysine (K) at amino acid position 17 results in a constitutively active form of the enzyme that is no longer dependent on activation of upstream components of the pathway. The AKT1 E17K mutation has been found in various cancers, including lung and breast

2. The PIK3CA gene encodes the p110a subunit of phosphatidyl 3-kinase, a lipid kinase involved in cell growth, proliferation, motility and survival. It has a central role in the PI3K-AKT-mTOR pathway. Somatic mutations in the PIK3CA gene have been implicated in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.

3. EntroGen BRCA Complete™ is a complete NGS solution from DNA to interpreting sequencing data that detects germline and somatic BRCA1/BRCA2 mutations with comprehensive coverage, specificity, and high sensitivity. BRCA Complete™ is compatible with blood, fresh frozen, and formalin fixed paraffin embedded (FFPE) samples. Allele dropouts and off-target reads are reduced through a tiled amplicon PCR approach coupled with a high fidelity polymerase for target enrichment. Reagents for library preparation and clean-up are included in BRCA Complete™ as well as a local, user-friendly software designed to reduce the bioinformatics burden on the end-user by calling clinically relevant mutations directly from VCF files. Sample and library quality control assays (DNA Fragmentation Quantification assay, Library Quantification Kit for Illumina®) are available to ensure high quality data without the need for repeat sequencing.

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