Entrogen's Real Time PCR Kits for Breast Cancer are available as following three categories :
AKT E17K Mutation Analysis Kit
For Real-Time PCR
PIK3CA Mutation Analysis Kit
For Real-Time PCR
For Illumina Platforms
1. AKT1 is a member of serine-threonine kinases that play a key role in various cellular processes including growth, proliferation, survival and angiogenesis. AKT1 is a downstream mediator of PI3 kinase. A somatic mutation that substitutes glutamic acid (E) with lysine (K) at amino acid position 17 results in a constitutively active form of the enzyme that is no longer dependent on activation of upstream components of the pathway. The AKT1 E17K mutation has been found in various cancers, including lung and breast
2. The PIK3CA gene encodes the p110a subunit of phosphatidyl 3-kinase, a lipid kinase involved in cell growth, proliferation, motility and survival. It has a central role in the PI3K-AKT-mTOR pathway. Somatic mutations in the PIK3CA gene have been implicated in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.
3. EntroGen BRCA Complete™ is a complete NGS solution from DNA to interpreting sequencing data that detects germline and somatic BRCA1/BRCA2 mutations with comprehensive coverage, specificity, and high sensitivity. BRCA Complete™ is compatible with blood, fresh frozen, and formalin fixed paraffin embedded (FFPE) samples. Allele dropouts and off-target reads are reduced through a tiled amplicon PCR approach coupled with a high fidelity polymerase for target enrichment. Reagents for library preparation and clean-up are included in BRCA Complete™ as well as a local, user-friendly software designed to reduce the bioinformatics burden on the end-user by calling clinically relevant mutations directly from VCF files. Sample and library quality control assays (DNA Fragmentation Quantification assay, Library Quantification Kit for Illumina®) are available to ensure high quality data without the need for repeat sequencing.