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High Throughput Fermentation in Microliter Scale


These bioprocesses must be grown quick and at the most extreme efficiency. Up to now, a few micro bioreactors were developed to satisfy these requests and to work with facilitate sample processing. One dominating response stage is the shaken microtiter plate (MTP), which gives high throughput at insignificant costs in time, money and work exertion. By taking this basic and proficient microbioreactor cluster, another online based monitoring method for biomass and fluorescence, called BioLector, has been recently developed. The blend of high-throughput and high data content makes the BioLector an integral asset in bioprocess advancement. All things considered, the adaptability of results from the small size to the lab or significantly bigger scales is vital for short development times. Accordingly, engineering parameters in regards to the reactor plan and its operation conditions play a significant role even on a micro-scale. To assess the scale-up from a microtiter plate scale (200 μL) to a blended tank fermenter scale (1.4 L), two standard microbial articulation system, Escherichia coli and Hansenula polymorpha were aged in equal at the two scales and looked at about the biomass and protein development.

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