Fluorescence microscopy, with its major impact on biomedical research over past several decades, the resolution barrier (Diffraction limit) inherent in light microscopy restricts the ability to differentiate between objects closer together than ∼250 nm laterally and >450–700 nm axially preventing the true sizing of structures smaller than this limit. The optical techniques used to resolve the structures beyond the diffraction limit, which was previously thought to be impenetrable are called Super-resolution microscopy(SRM) techniques.
The impact of super-resolution microscopy is rapidly expanding as commercial super-resolution microscopes become available. However, super-resolution microscopes are not based on a single technology and the differences between the individual technologies can influence how suited each approach is to address a specific cell biological question. Few of the well known and commonly used techniques are STED,SIM,STORM,PALM etc globally.
DSS Imagetech, being a well known supplier of microscopes and digital solutions in India has already been actively promoting major super-resolution microscopy techniques (STED) and sold few of the systems in the country in the past few years.