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Digital MLPA Assay

MRC HOLLAND Digital MLPA Assay in India

Digital MLPA Assay

SALSA® digital Multiplex Ligation-dependent Probe Amplification (digitalMLPA) is a multiplex PCR followed by Illumina sequencing-based amplicon quantification, for the detection of copy number variations (CNV) and specific point mutations. 

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Features & Benefits

  • Reliable: proven copy number detection technology
  • Robust: only 20 ng of sample DNA needed; uniform coverage
  • Quality control: control probes included in each reaction
  • Simple: easy hands-on steps and no library quantification needed
  • Cost-effective: up to 1000 probes in one reaction
  • Sample certainty: SNV probes present to distinguish samples
  • Easy analysis: user-friendly analysis with free Coffalyser digitalMLPA software
  • Extensive multiplexing: Suitable on Illumina platforms - iSeq 100, MiSeq System (v3 chemistry), MiniSeq System, NextSeqTM 500/550 System, HiSeq 2000/2500"

Product Details

digitalMLPA amplifies ligated probes with a universal primer pair enabling unbiased amplification. With digitalMLPA, up to 1000 unique sequences can be detected and quantified in a single reaction.

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