Entrogen RT PCR Kits for EGFR

More Views

Epidermal growth factor receptor (EGFR) is a membrane protein that plays a central role in sending signals that promote cell growth and proliferation. Its tyrosine kinase (TK) domain triggers several downstream effectors that lead to the activation of the Ras-Raf-MAPK track. Overexpression and oncogenic mutations that constitutively activate the TK domain of EGFR have been found in various solid tumours. Additionally, excessive activation of EGFR is associated with advanced stages of cancer and a poor prognosis.

Add to Shortlist

Features & Benefits

1% Limit of Detection (LOD)
Highly sensitive and specific
Works with FFPE, fresh frozen and FNA samples for EGFR Mutation Analysis Kits and plasma for ctEGFR kit.
Simple setup and interpretation
Complementary spreadsheet for automated analysis for EGFR Mutation Analysis Kits
Detects low mutant copy number
Low input requirement
Easy setup & interpretation

Product Details

Tumour tissue from non-small cell lung cancer patients is routinely tested for the existence of somatic mutations in the epidermal growth factor receptor (EGFR) to predict response to EGFR tyrosine kinase inhibitor (TKI) therapy. However, in a significant number of cases, tumour tissue is not available in sufficient amounts for accurate molecular testing. Studies have demonstrated the utility of cell-free tumour DNA (ctDNA) from plasma as an alternative source of genomic material for the detection of sensitizing and resistance mutations in lung cancer.

Glioblastoma multiforme (GBM) is an antagonistic form of brain cancer that accounts for around 45% of malignant brain tumours. Advancements in molecular profiling and precision medicine have the potential to improve the diagnosis, treatment and prognosis of GBM patients. EGFRvIII, the most common EGFR mutation in GBM, is found in approximately 60-70% of EGFR-amplified primary GBM tumours and is a potential target for experimental treatments.

Entrogen has many solutions for EGFR mutations which are RT-PCR based:

EGFR-RT52

EGFR Mutation Analysis Kit for Real-Time PCR is a 52 reactions RT PCR Assay that uses mutant-specific probes to identify the presence of EGFR mutations. Exons covered: 18, 19, 20, 21 For detailed Exon Coverage, please contact us. This kit needs a real-time PCR instrument capable of detecting FAM & VIC fluorescent probes simultaneously.

EGFRX-RT52

EGFRX Mutation Analysis Extension Kit is a 52 reactions RT-PCR extended coverage assay which uses mutant-specific probes to identify the presence of EGFR mutations. Extended coverage includes exons covered: 18 & 20. 18 (E709, E709A, E709D (c.2127 A>C), E709D (c.2127 A>T), E709G, E709K, E709Q, E709V) 20 (L858M, L861R) This kit requires a real-time PCR instrument capable of detecting FAM & VIC fluorescent probes simultaneously. ctEGFR-48: ctDNA EGFR Mutation Detection Kit is a 48 reactions RT-PCR Assay that is a non-invasive, ultra-sensitive test to accurately detect the presence of EGFR activating mutations in ctDNA extracted from plasma. The test identifies the most common somatic mutations in exon 18-21 in EGFR, including the C797S resistance mutation. This kit needs a real-time PCR instrument capable of detecting FAM, VIC, ROX and CY5 fluorescent probes simultaneously.

EGFRV3-RT42

EGFRvIII Detection Kit is a 42 reactions RT-PCR assay to detect this mutation in total RNA isolated from tumour biopsies in a single step process (RNA to results in one step). This kit requires a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes EntroGen’s EGFR mutation analysis kit requires 40-80 nanograms of genomic DNA (5-10 nanograms per reaction) and can detect a mutation load of less than 1%. This sensitivity greatly depends on the extent of fragmentation and quality of the isolated DNA.