In situ hybridization (ISH) techniques are used to localize specific nucleic acid sequences within the DNA in cells in tissues or cytological preparations, on chromosomes or in whole mounts. We provide a wide variety of probes and kits aimed at the most predominant molecular tests performed in anatomic pathology. The IQFISH hybridization buffer is non-toxic and allows genomic DNA probe hybridization to be performed outside a fume hood in just 60-120 minutes. The short hybridization time results in a turnaround time of about 4 hours for a complete FISH staining from deparaffinization to mounting. Hybridizer is a hands-free denaturation and hybridization instrument designed for semi-automated, slide-based fluorescence and chromogenic in situ hybridization (FISH and CISH, respectively) on histology and cytology samples.
Hybridization techniques are used to localize and identify specific nucleic acid sequences. Initially used as a research tool, the development of non-radioactive probe labeling and detection systems has made this technology available to a wide variety of routine applications. Traditionally either cloned probes or synthesized oligonucleotide probes have been used for hybridization. We successfully introduced the peptide nucleic acid (PNA) probe, a nucleotide analogue capable of binding to DNA/RNA in a sequence-specific manner obeying the Watson-Crick base pairing rules. In this section, you will find molecular pathology products which are not intended for FISH. The probes are either visualized using the GenPoint CSA System, In Situ Hybridization Detection System, PNA ISH Detection System or Chemiluminescent System for Nucleic Acid Blotting.
The following Labeled Probes & Detection Systems are available:
- GenPoint™; Amplified Signal Detection System
- Human Papillomavirus DNA Probes
- PNA Probes & Detection System
- Standard Nucleic Acid Detection System