DNA Flow Technology
DNA Flow technology is an inventive innovation of mechanized automated flow-through reverse hybridization on a microarray of a permeable layer that contains explicit oligonucleotides to every marker. The target DNAs experience the membrane by a vertical vacuum flow, which encourages the interaction with their particular and integral DNA probes immobilized on the porous membrane, in a three-dimensional environment, as opposed to the conventional dot-blot technology, in which hybridization happens latently. Therefore, the cycle accelerates, and the affectability expands, reducing the reaction volumes and the absolute handling time from a few hours to a few minutes. It gives the platform for fast analyses in membrane-based reverse dot blot hybridization procedure. DNA target molecules are commanded to complementary acquisition probes immobilised in the matrix pores. The reaction between the target DNA and probes in a three-dimensional environment results in high signal intensity in a very short period.
When the binding between the particular amplicons and their relating tests has occurred, the signal is visualized through an immunoenzymatic colourimetric reaction with Streptavidin−Alkaline Phosphatase and the NBT/BCIP chromogen, creating insoluble precipitates in the membrane in those situations where there has been hybridization. The cycle is done consequently in the hybriSpot platforms and the outcomes are examined with the hybriSoft software.
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hybriSpot 12 is a semiautomatic DNA Flow hybridization system for 12 samples. It is completely guided by the display which provides precise instructions during the analysis process.
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hybriSpot 24 is an Automatic DNA Flow hybridization system for 24 samples, completely guided by hybriSoft software
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