Melanoma is the fifth most common malignancy in the United States. Approximately 70% of melanoma cases harbor recurrent somatic mutations that aberrantly activate growth and proliferation pathways and lead to tumorigenesis. The most common mutations found in melanoma are BRAF mutations occurring at codon 600 in approximately 40-60%, followed by NRAS mutations in 15-25% and c-Kit in 2-8% of cases. BRAF mutations have been associated with increased sensitivity to BRAF inhibitors. While there are no approved anti-NRAS targeted therapies, NRAS-mutated melanomas have demonstrated increased sensitivity to various RTK and MEK inhibitors.
Available kits are as follows :
|S.No.||Product Name||Catalog Number||Platform|
|1||BRAF Codon 600 Mutation Analysis Kit II||BRAFX-RT64||For Real-Time PCR|
|2||NRAS Mutation Analysis Kit||NRAS-RT50||For Real-Time PCR|
|3||c-Kit Mutation Detection Kit||CKIT-RT44||For Real Time PCR|
|4||ctDNA BRAF V600 Mutation Detection Kit||CTBRAF-48||For Real-Time PCR|
The above kits are polymerase chain reaction (PCR)-based assays that use allele-specific primers in a multiplex reaction to identify the presence of BRAF, NRAS, and c-Kit mutations. The assays work by amplifying mutant-specific sequences in samples that contain a mixture of mutant and wild-type DNA and rely on fluorescent probes for detection. Each reaction contains primer sets and probes for detection of the mutations, as well as an endogenous control gene.
The testing procedure involves three (3) simple steps:
1. Isolation of DNA from tumor biopsies, paraffin-embedded sections (FFPE), fresh frozen tumors, or plasma (for cell-free kits).
2. Amplification using the provided reagents in the kit.
3. Data analysis and interpretation using the real-time PCR software or provided analysis worksheet.
All kits require a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes. Additionally, the c-Kit Mutation Detection Kit requires capability to detect ROX and CY5 fluorescent probes.
All reagents required for PCR amplification/detection, as well as validated reaction controls are included. Columns and reagents for DNA isolation are not included.