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SALSA MLPA Reagent Kits for Endocrine & Reproductive

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SALSA MLPA Reagent Kits for Endocrine & Reproductive
MLPA (Multiplex Ligation-dependant Probe Amplification (MLPA) is a multiplex PCR method that enables the copy number variation (CV) detection of up to 60 DNA sequences in a single reaction. 96 DNA samples can be handled simultaneously with results being available within 24 hours
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Features & Benefits

  • Endocrine & Reproductive disease detection
  • Simultaneous Detection of copy number, methylation and known point mutation
  • Low Input - Requires only 50 ng of DNA
  • Reproducible - High concordance with other techniques
  • Time Efficient - Results available within 24 hrs.
  • High Throughput - Can detect up to 60 sequences in 96  DNA samples, in one reactions
  • Cost Effective

Product Details

MLPA® is the gold standard for DNA copy number quantification and is used to study both hereditary disorders and tumours. MLPA can also be applied to investigate the methylation status of DNA sequences. MRC-Holland, the inventor and manufacturer of MLPA, offers 400 different MLPA panels. MLPA (Multiplex Ligation-dependant Probe Amplification (MLPA) is a multiplex PCR method that enables the copy number variation (CV) detection of up to 60 DNA sequences in a single reaction. 96 DNA samples can be handled simultaneously with results being available within 24 hours.


Components provided per SALSA MLPA Reagent Kit  



  • SALSA MLPA Buffer (yellow cap)

  • SALSA Ligase-65 (green cap)

  • Ligase Buffer A (transparent cap)

  • Ligase Buffer B (white cap)

  • SALSA PCR Primer Mix (brown cap)

  • SALSA Polymerase (orange cap) 


MLPA has the following solutions for Endocrine & Reproductive diseases:
































































































































































PRODUCT NAME APPLICATION REGION
ME031-GNAS Albright hereditary osteodystrophy (AHO), Pseudohypoparathyroidism (PHP) GNAS 20q13.32
ME033-TNDM Transient neonatal diabetes mellitus 6q24
P017-MEN1 Multiple endocrine neoplasia MEN1 11q13
P049-SLC6A8 - ABCD1 Creatine transporter, x-linked SLC6A8, Xq28
P050-CAH Congenital Adrenal Hyperplasia CYP21A2 6p21.3
P074-AR Androgen insensitivity syndrome (AIS) AR Xq12
P117-ABCC8 Hyperinsulinemic hypoglycemia, familial (HHF1) ABCC8 11p15.1
P133-Kallmann-2 Kallmann syndrome FGFR1 8p11.2, GNRHR 8p21, KISSR1 19p13.3 , GNRH1, NELF 9q34.3
P177-CASR Hypocalciuric Hypercalcemia, familial CASR 3q13
P185-Intersex Sex determination SOX9 17q24.3; NR5A1 9q33; WNT4 1p36.12; CXorf21 & NROB1 Xp21.2
P216-GHD mix1 Growth Hormone Deficiency (GHD) GH1, LHX4, POU1F1, HESX1, PROP1, GHRHR, LHX3
P217-IGF1R Insulin-like growth factor 1 receptor (IGF1R) IGFR1, IGFBP3.
P220-Obesity Obesity LEPR, POMC, LEP, SIM1, MC3R, MC4R
P224-PPARG Obesity, Diabetes mellitis type II, Familial partial lipodystrophy III, Colon cancer PPARG 3p25.2
P226-SDH Paragangliomas (PGL) SDHD 11q23.1, SDHB 1p36.1, SDHC 1q23.3
P234-GATA3 - GATA4 Cardiac septal defects GATA4 8p23, GATA3 10p15
P241-MODY Maturity-Onset Diabetes of the Young (MODY) MODY 1, 2, 3; 5
P244-AIP-MEN1-CDKN1B Multiple endocrine neoplasia (MEN) AIP 11q13, MEN1 11q13, CDKN1B 12p13
P251-NB mix 1 Neuroblastoma 1p36, 3p22/3p21.3 and 11q23
P252-NB mix 2 Neuroblastoma 2p24.1/MYCN, 2q33, 17p13/TP53, 17q
P253-NB mix 3 Neuroblastoma Chromosomes 4, 7, 9, 12, 14
P312-POR Congenital adrenal hyperplasia (CAH) P450 oxireductase gene (POR)
P319-Thyroid Thyroid dysgenesis TPO, PAX8, FOXE1, NKX2-1, TSHR
P334-Gonadal Gonadal Development Disorder DMRT1, CYP17A1, SRD5A2, HSD17B3
P357-MODY mix-2 Maturity-Onset Diabetes of the Young (MODY) MODY 4; 6-10
P360-Y-Chromosomebasic research Y-Chromosome Microdeletions Chromosome Y
P429-SDHA-MAX Paraganglioma and pheochromocytoma 5p15.3 and 14q23.3
P461-DIS Deafness Infertility Syndrome (DIS); non-syndromic hearing loss 15q15.3; 16p12.2
P463-MRKH Mayer-Rokitansky-Küster-Hauser Syndrome 16p11.2; 17q12; 22q11.21
P466-CDC73 HPT jaw tumour syndrome, famial hyperparathyroidism, parathyroid carcinoma CDC73
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