• ABOUT
  • THE GROUP
  • NEWS & MEDIA
  • EVENTS
  • CAREER
  • CONTACT US
  • 0

SALSA MLPA Reagent Kits for Gastrointestinal & Liver

More Views

SALSA MLPA Reagent Kits for Gastrointestinal & Liver
MLPA (Multiplex Ligation-dependant Probe Amplification (MLPA) is a multiplex PCR method that enables the copy number variation (CV) detection of up to 60 DNA sequences in a single reaction. 96 DNA samples can be handled simultaneously with results being available within 24 hours.
Read More
Add to Shortlist

Features & Benefits

  • Detection of gastrointestinal & liver diseases
  • Simultaneous Detection of copy number, methylation and known point mutation
  • Low Input - Requires only 50 ng of DNA
  • Reproducible - High concordance with other techniques
  • Time Efficient - Results available within 24 hrs.
  • High Throughput - Can detect up to 60 sequences in 96  DNA samples, in one reactions
  • Cost Effective

Product Details

MLPA® is the gold standard for DNA copy number quantification and is used to study both hereditary disorders and tumours. MLPA can also be applied to investigate the methylation status of DNA sequences. MRC-Holland, the inventor and manufacturer of MLPA, offers 400 different MLPA panels. MLPA (Multiplex Ligation-dependant Probe Amplification (MLPA) is a multiplex PCR method that enables the copy number variation (CV) detection of up to 60 DNA sequences in a single reaction. 96 DNA samples can be handled simultaneously with results being available within 24 hours.


Components provided per SALSA MLPA Reagent Kit  



  • SALSA MLPA Buffer (yellow cap)

  • SALSA Ligase-65 (green cap)

  • Ligase Buffer A (transparent cap)

  • Ligase Buffer B (white cap)

  • SALSA PCR Primer Mix (brown cap)

  • SALSA Polymerase (orange cap) 


MLPA has the following solutions for Gastrointestinal & Liver:



























































































































































PRODUCT NAME APPLICATION REGION
ME011-MMR Mismatch repair genes (MMR) MLH1, MSH2, MSH6, MLH3, MSH3, PMS2, MGMT
ME042-CIMPbasic research CpG Island Methylator Phenotype Various
P003-MLH1/MSH2CE Lynch Syndrome MLH1 3p22.2, MSH2 2p21
P008-PMS2 Lynch Syndrome PMS2 7p22
P043-APC Hereditary Polyposis Colon Cancer APC, 5q21-q22
P072-MSH6 Lynch Syndrome MSH6
P083-CDH1 Hereditary diffuse gastric cancer CDH1 at 16q22.1
P098-Wilson Wilson disease ATP7B 13q14.3
P101-STK11 Peutz-Jeghers syndrome (PJS) STK11 19p13.3
P109-ABCB4 Drug-transport pump ABCB4 7q21,1
P146-CRC GAINbasic research Colon cancer Various
P158-JPS Juvenile polyposis syndrome (JPS) BMPR1A 10q22, SMAD4 18q21, PTEN 10q23
P169-Hirschsprung-1 Hirschsprung disease, or Aganglionic Megacolon RET 10q11.2, ZFHX1B 2q22.3, EDN3 20q13.3 and GDNF 5q13.2
P184-JAG1 Alagille Syndrome (AGS) JAG1 20p12.2
P242-Pancreatitis Hereditary Pancreatitis (HP) PRSS1 7q34, SPINK1 5q32
P248-MLH1-MSH2 Confirmation Lynch Syndrome MLH1 3p21.3, MSH2 2p21
P318-Hirschsprung-2 Hirschsprung disease PHOX2B, GFRA3, GFRA2, GFRA1, EDNRB, NRTN, PSPN, SOX10
P341-PKHD1 mix 1 Autosomal Recessive Polycystic Kidney Disease (ARPKD) PKHD1
P342-PKHD1 mix 2 Autosomal Recessive Polycystic Kidney Disease (ARPKD) PKHD1
P347-Hemochromatosis Hereditary hemochromatosis (HH) HFE, SLC40A1, TFR2, HFE2, HAMP
P378-MUTYH Colon cancer, stomach cancer (hereditary) 1p34 and 15q13
P393-SLC26A3 Congenital Chloride Diarrhoea SLC26A3 7q31.1
P394-MYO5B Microvillus Inclusion Disease (MVID) MYO5B 18q21.1
P411-Porphyria mix 1 Porphyrias ALAD, HMBS, PPOX
P412-Porphyria mix 2 Porphyrias FECH, UROS, UROD, CPOX
P413-CRC LOSSbasic research Colorectal adenoma/carcinoma 8p, 15q, 17p, 18q
P438-HLA Celiac Disease, HLA-DQ2.5 HLA-DQ8 HLA-DQ2.2 6p21.32
P458-Gastric cancer Gastric cancer various
P459-SERPINA1 Alpha-1-antitrypsin (AAT)-deficiency 14q32.13
Read More