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SALSA MLPA Reagent Kits for Hearing Impairment

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SALSA MLPA Reagent Kits for Hearing Impairment
MLPA (Multiplex Ligation-dependant Probe Amplification (MLPA) is a multiplex PCR method that enables the copy number variation (CV) detection of up to 60 DNA sequences in a single reaction. 96 DNA samples can be handled simultaneously with results being available within 24 hours.
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Features & Benefits

  • Hearing Impairment solutions
  • Simultaneous Detection of copy number, methylation and known point mutation
  • Low Input - Requires only 50 ng of DNA
  • Reproducible - High concordance with other techniques
  • Time Efficient - Results available within 24 hrs.
  • High Throughput - Can detect up to 60 sequences in 96  DNA samples, in one reactions
  • Cost Effective

Product Details

MLPA® is the gold standard for DNA copy number quantification and is used to study both hereditary disorders and tumours. MLPA can also be applied to investigate the methylation status of DNA sequences. MRC-Holland, the inventor and manufacturer of MLPA, offers 400 different MLPA panels. MLPA (Multiplex Ligation-dependant Probe Amplification (MLPA) is a multiplex PCR method that enables the copy number variation (CV) detection of up to 60 DNA sequences in a single reaction. 96 DNA samples can be handled simultaneously with results being available within 24 hours.


Components provided per SALSA MLPA Reagent Kit  



  • SALSA MLPA Buffer (yellow cap)

  • SALSA Ligase-65 (green cap)

  • Ligase Buffer A (transparent cap)

  • Ligase Buffer B (white cap)

  • SALSA PCR Primer Mix (brown cap)

  • SALSA Polymerase (orange cap) 


MLPA has the following solutions for Hearing Impairment:


























































































PRODUCT NAME APPLICATION REGION
P153-EYA1 Branchio-oto-renal dysplasia syndrome (BOR) EYA1 8q13.3.
P163-GJB-WFS1 Hearing loss GJB3 1p34.3 (connexin 31), WFS1 4p16.1 (Wolframin)
P186-PAX3-MITF-SOX10 Waardenburg syndrome (WS) type II, WS1, WS3 PAX3 2q35, MITF 3p14, SOX10 22q13.1
P191-Alport-mix1 Alport syndrome, Hereditary Nephritis COL4A5 Xq22
P192-Alport-mix2 Alport syndrome, Hereditary Nephritis COL4A5 Xq22
P234-GATA3 - GATA4 Cardiac septal defects GATA4 8p23, GATA3 10p15
P280-SLC26A4 Pendred syndrome SLC26A4 7q31
P292-PCDH15 Usher syndrome PCDH15 10q21.1
P310-TCOF1 Treacher Collins-Franceschetti 1 TCOF1
P361-USH2A mix 1 Usher syndrome USH2A 1q41
P362-USH2A mix 2 Usher syndrome USH2A 1q41
P381-COL11A1 mix 1 Marshall, type II Stickler syndromes COL11A1
P382-COL11A1 mix 2 Marshall, type II Stickler syndromes COL11A1
P439-COL4A3 Alport Syndrome 02q36.3
P444-COL4A4 Alport Syndrome 2q36.3
P461-DIS Deafness Infertility Syndrome (DIS); non-syndromic hearing loss 15q15.3; 16p12.2
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