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SALSA MLPA Reagent Kits for Neurological

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SALSA MLPA Reagent Kits for Neurological
MLPA (Multiplex Ligation-dependant Probe Amplification (MLPA) is a multiplex PCR method that enables the copy number variation (CV) detection of up to 60 DNA sequences in a single reaction. 96 DNA samples can be handled simultaneously with results being available within 24 hours.
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Features & Benefits

  • Detection of Neurological Disorders
  • Simultaneous Detection of copy number, methylation and known point mutation
  • Low Input - Requires only 50 ng of DNA
  • Reproducible - High concordance with other techniques
  • Time Efficient - Results available within 24 hrs.
  • High Throughput - Can detect up to 60 sequences in 96  DNA samples, in one reactions
  • Cost Effective

Product Details

MLPA® is the gold standard for DNA copy number quantification and is used to study both hereditary disorders and tumours. MLPA can also be applied to investigate the methylation status of DNA sequences. MRC-Holland, the inventor and manufacturer of MLPA, offers 400 different MLPA panels. MLPA (Multiplex Ligation-dependant Probe Amplification (MLPA) is a multiplex PCR method that enables the copy number variation (CV) detection of up to 60 DNA sequences in a single reaction. 96 DNA samples can be handled simultaneously with results being available within 24 hours.

Components provided per SALSA MLPA Reagent Kit  

  • SALSA MLPA Buffer (yellow cap)

  • SALSA Ligase-65 (green cap)

  • Ligase Buffer A (transparent cap)

  • Ligase Buffer B (white cap)

  • SALSA PCR Primer Mix (brown cap)

  • SALSA Polymerase (orange cap) 

MLPA has the following solutions for Neurological:

ME011-MMR Mismatch repair genes (MMR) MLH1, MSH2, MSH6, MLH3, MSH3, PMS2, MGMT
ME029-FMR1/AFF2 Fragile X FMR1, AFF2
P015-MECP2 RETT syndrome MECP2, Xq28
P022 -PLP1 Pelizaeus-Merzbacher disease (PMD) PLP1 Xq22
P025-Canavan Canavan disease ASPA, 17p13
P041-ATM-1 Ataxia-Telangiectasia (AT) ATM 11q23
P042-ATM-2 Ataxia-Telangiectasia (AT) ATM 11q23
P044-NF2 Neurofibromatosis type 2 (NF2) NF2 22q12
P046-TSC2 Tuberous Sclerosis TSC2 16p13.3
P049-SLC6A8 - ABCD1 Creatine transporter, x-linked SLC6A8, Xq28
P051-Parkinson mix 1 Parkinson disease, familial PARK2 6q25.2, SNCA 4q21, Pink1, Park7 1p36
P052-Parkinson mix 2 Parkinson disease, familial PARK2 6q25.2, UCHL1 4p14, GCH1 14q22.1, LRRK2 12q12
P059-Dystonia Dystonia PRKRA; THAP1; TOR1A; ATP1A3
P061-Lissencephalyimproved Lissencephaly LIS1, DCX, POMT1, POMGnT1, FLNA
P063-FHIT-WWOX Diverse tumour types, epileptic encephalopathy FHIT (3p14.2) and WWOX (16q23.1)
P071-LMNB1-PLP1-NOTCH3 Leukodystrophy LMNB1 5q23.2, PLP1 Xq22.2, NOTCH3 19p13.12
P075-TCF4-FOXG1 Pitt-Hopkins syndrome, Congential variant of Rett syndrome TCF4, FOXG1
P081-NF1 mix 1 Neurofibromatosis NF1 17q11.2
P082-NF1 mix 2 Neurofibromatosis NF1 17q11.2
P088-Oligodendroglioma 1p-19q Oligodendroglioma 1p, 19q, IDH1/2, CDKN2A/2B
P098-Wilson Wilson disease ATP7B 13q14.3
P099-GCH1-TH-SGCE Dopa-responsive dystonia, Segawa disease; Myoclonus-dystonia syndrome TH 11p15.5, GCH114q22, SGCE 7q21
P103-DPYD Dihydropyrimidine dehydrogenase (DPYD) deficiency DPYD 1p22
P105-Glioma-2 Glioma, malignant EGFR, TP53, PTEN + various other
P107-Neurometabolic Neurometabolic disorders ASPA, MLC1, MLYCD, D2HGDH, L2HGDH
P120-PANK2/PLA2G6 Neurodegeneration with brain iron accumulation (NBIA) PANK2 20q13, PLA2G6 22q13
P122-NF1-area Neurofibromatosis NF1 17q11.2
P124-TSC1 Tuberous Sclerosis TSC1 9q34
P130-CCM mix-1 Cerebral Cavernous Malformations (CCM) CCM 7q21
P131-CCM mix-2 Cerebral Cavernous Malformations (CCM) CCM 7q21
P137-SCN1A Epilepsy SCN1A 2q24.3
P138-SLC2A1-STXBP1 Glut1 deficiency syndrome SLC2A1, STXBP1
P165-HSP Spastic paraplegia, hereditary (HSP) SPG3A 14q21, SPAST 2p22
P166-KCNQ2 Benign familial neonatal convulsion (BFNC) KCNQ2 20q13.33
P170-APP Alzheimer disease, early-onset APP 21q21.3
P187-HPE Holoprosencephaly (HPE) PTCH, SHH, ZIC2, SIX3, TGIF TMEM1, FBXW11
P189-CDKL5 Rett syndrome, atypical CDKL5 Xp22, NTNG1 1p13.3, ARX Xp21.3
P197-KCNQ3 Neonatal convulsions type 2, familial (BFNC2), Epilepsy benign neonatal type 2 (EBN2) KCNQ3 8q24.22; CHRNA4 20q13.33; EPM2A 6q24.3; NHLRC1 6p22.3; CHRNB2 1q21.3; KCNQ1 11p55.5-4
P199-HEXA Tay-Sachs Disease HEXA 15q23
P211-HSP region Hereditary Spastic Paraplegias (HSPs) region SPAST 2p22, NIPA1 15q11
P213-HSP mix-2 Hereditary spastic paraplegias (HSPs) REEP1 (SPG31) 2p11.2, SPG7 16q24.3
P226-SDH Paragangliomas (PGL) SDHD 11q23.1, SDHB 1p36.1, SDHC 1q23.3
P251-NB mix 1 Neuroblastoma 1p36, 3p22/3p21.3 and 11q23
P252-NB mix 2 Neuroblastoma 2p24.1/MYCN, 2q33, 17p13/TP53, 17q
P253-NB mix 3 Neuroblastoma Chromosomes 4, 7, 9, 12, 14
P254-PSEN1 Alzheimer's disease (AD) PSEN1 14q24.2
P267-Dandy-Walker Dandy-Walker Malformation (DWM) ZIC1, ZIC4 3p2, VLDLR 9p24
P274-SLC6A5/GLRA1/GLRB Startle disease, Hyperekplexia GLRB 4q32.1, GLRA1 5q33.1, SLC6A5 11p15.1
P275-MAPT-GRN Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration, progressive supranuclear palsy MAPT 17q21
P279-CACNA1A Episodic ataxia 2, Familial hemiplegic migraine CACNA1A
P301-Medulloblastoma mix 1 Medulloblastoma Chr. 6, Chr. 14, Chr. 16, Chr. 17.
P302-Medulloblastoma mix 2 Medulloblastoma Chr. 2, Chr. 3, Chr. 7, Chr. 9
P303-Medulloblastoma mix 3 Medulloblastoma Chr. 1, Chr. 4, Chr. 5, Chr. 8, Chr. 10, Chr. 20
P306-SPG11 Hereditary spastic paraplegia (HSP or SPG) SPG11 or KIAA1840 15q21.1
P307-SEPT9 Hereditary neuralgic amyotrophy (HNA) SEPT9 17q25.3
P316-Recessive Ataxias Recessive Ataxias SETX, APTX, FXN.
P326-LARGE Walker-Warburg Syndrome LARGE, FKTN, POMT2
P330-PCDH19 Epilepsy (EFMR) PCDH19
P336-UBE3A Angelman UBE3A, MTHFR, GABRB3
P337-TSC2 Tuberous sclerosis TSC2 gene, 16p13.3
P339-SHANK3 Autism Spectrum Disorder SHANK3 22q13.33
P343-Autism-1 Autism 15q11-13: UBE3A, GABRB3; 16p11; SHANK3 22q13
P348-ATP1A2-CACNA1Aimproved Migraine 1q23, 19p13
P355-Microcephaly Primary Microcephaly MCPH1, ASPM, CDK5RAP2, CENPJ, STIL
P370-BRAF-IDH1-IDH2basic research Gliomas BRAF, IDH1, IDH2, FGFR1, MYB, MYBL1, CDKN2A
P388-AGS Aicardi-Goutieres Syndrome RNASEH2A, B & C genes, TREX1, SAMHD1
P395-MEF2C-FOXG1 mental retardation MEF2C 5q14.3, FOXG1 14q12
P396-SHANK2 Autism-spectrum disorder SHANK2 11q13.3
P398-CASK X-linked mental retardation and MICPCH CASK
P408-ADLTE-LGI1 Autosomal Dominant Lateral Temporal Lobe Epilepsy, ADLTE ADAM22, GPR98, KCNA1, KCNA4, KCNAB1, LGI1, PDYN
P410-GRIN2A GRIN2B epilepsy, mental retardation 16p13.2 GRIN2A, 12p13.1 GRIN2B
P411-Porphyria mix 1 Porphyrias ALAD, HMBS, PPOX
P412-Porphyria mix 2 Porphyrias FECH, UROS, UROD, CPOX
P427-PDHA1 Leigh Syndrome X-chromosome
P429-SDHA-MAX Paraganglioma and pheochromocytoma 5p15.3 and 14q23.3
P441-SACS ARSACS (autosomal recessive spastic ataxia of Charlevoix-Saguenay) 3q12.12
P446-GALC Krabbe Disease 14q31.3
P447-HPRT1 Lesch-Nyhan syndrome Xq26.1
P455-LZTR1 Schwannomatosis 22q11.21
P470-NCL Neuronal Ceroid Lipofuscinoses/Batten Disease 01p34.2, 08p23.3, 11p15.4, 15q23, 16p11.2
P471-EOFAD Early-Onset Familial Alzheimer's Disease (EOFAD) 01q42.13; 14q24.2; 21q21.3
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