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SALSA MLPA Reagent Kits for Newborn

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SALSA MLPA Reagent Kits for Newborn
MLPA (Multiplex Ligation-dependant Probe Amplification (MLPA) is a multiplex PCR method that enables the copy number variation (CV) detection of up to 60 DNA sequences in a single reaction. 96 DNA samples can be handled simultaneously with results being available within 24 hours.
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Features & Benefits

  • Simultaneous Detection of copy number, methylation and known point mutation
  • Low Input - Requires only 50 ng of DNA
  • Reproducible - High concordance with other techniques
  • Time Efficient - Results available within 24 hrs.
  • High Throughput - Can detect up to 60 sequences in 96  DNA samples, in one reactions
  • Cost Effective

Product Details

MLPA® is the gold standard for DNA copy number quantification and is used to study both hereditary disorders and tumours. MLPA can also be applied to investigate the methylation status of DNA sequences. MRC-Holland, the inventor and manufacturer of MLPA, offers 400 different MLPA panels. MLPA (Multiplex Ligation-dependant Probe Amplification (MLPA) is a multiplex PCR method that enables the copy number variation (CV) detection of up to 60 DNA sequences in a single reaction. 96 DNA samples can be handled simultaneously with results being available within 24 hours.


Components provided per SALSA MLPA Reagent Kit  



  • SALSA MLPA Buffer (yellow cap)

  • SALSA Ligase-65 (green cap)

  • Ligase Buffer A (transparent cap)

  • Ligase Buffer B (white cap)

  • SALSA PCR Primer Mix (brown cap)

  • SALSA Polymerase (orange cap) 


MLPA has the following solutions for Newborn:
















































































PRODUCT NAME APPLICATION REGION
P018-SHOXCE Idiopathic growth retardation SHOX-Xp22
P033-CMT1CE Charcot-Marie Tooth disease (CMT1), Hereditary Neuropathy with liability to Pressure Palsies (HNPP) CMT1/HNPP region, 17p12
P034-DMD-1CE Duchenne Muscular Dystrophy (DMD) DMD, Xp21.2
P035-DMD-2CE Duchenne Muscular Dystrophy (DMD) DMD, Xp21.2
P036-Subtelomeres Mix 1CE Subtelomeric testing All subtelomeres
P055-PAHCE Phenylketonuria PAH 12q23.2
P060-SMACE Spinal Muscular Atrophy (SMA) SMN1, SMN2, 5q13
P069-Subtelomeres Mix 2ACE Subtelomeric testing All subtelomeres
P070-Subtelomeres Mix 2BCE Subtelomeric testing All subtelomeres
P091-CFTRCE Cystic Fibrosis CFTR 7q31.2
P095-AneuploidyCE Down syndrome, Edwards syndrome, Patau syndrome Chr. 13, 18, 21, X, Y
P140-HBACE Thalassemias, Alpha HBA 16p
P250-DiGeorgeCE DiGeorge syndrome 22q11
P405-CMT1CE Charcot Marie Tooth Disease CMT/HNPP region at 17p12
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