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SALSA MLPA Reagent Kits for Skeletal & Connective Tissue

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SALSA MLPA Reagent Kits for Skeletal & Connective Tissue
MLPA (Multiplex Ligation-dependant Probe Amplification (MLPA) is a multiplex PCR method that enables the copy number variation (CV) detection of up to 60 DNA sequences in a single reaction. 96 DNA samples can be handled simultaneously with results being available within 24 hours.
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Features & Benefits

  • Skeletal & Connective Tissue Disorders
  • Simultaneous Detection of copy number, methylation and known point mutation
  • Low Input - Requires only 50 ng of DNA
  • Reproducible - High concordance with other techniques
  • Time Efficient - Results available within 24 hrs.
  • High Throughput - Can detect up to 60 sequences in 96  DNA samples, in one reactions
  • Cost Effective

Product Details

MLPA® is the gold standard for DNA copy number quantification and is used to study both hereditary disorders and tumours. MLPA can also be applied to investigate the methylation status of DNA sequences. MRC-Holland, the inventor and manufacturer of MLPA, offers 400 different MLPA panels. MLPA (Multiplex Ligation-dependant Probe Amplification (MLPA) is a multiplex PCR method that enables the copy number variation (CV) detection of up to 60 DNA sequences in a single reaction. 96 DNA samples can be handled simultaneously with results being available within 24 hours.


Components provided per SALSA MLPA Reagent Kit  



  • SALSA MLPA Buffer (yellow cap)

  • SALSA Ligase-65 (green cap)

  • Ligase Buffer A (transparent cap)

  • Ligase Buffer B (white cap)

  • SALSA PCR Primer Mix (brown cap)

  • SALSA Polymerase (orange cap) 


MLPA has the following solutions for Skeletal & Connective Tissue:



























































































































































PRODUCT NAME APPLICATION REGION
P018-SHOXCE Idiopathic growth retardation SHOX-Xp22
P065-Marfan-1 Marfan syndrome FBN1 15q21.1, TGFBR2 3p22
P066-Marfan-2 Marfan syndrome FBN1 15q21.1
P080-Craniofacial Craniofacial disorders FGFRs, TWIST, MSX2, ALX4, RUNX2
P092-ABCC6 Pseudoxanthoma elasticum ABCC6 16p13.11
P148-TGFBR1-TGFBR2 Aortic aneurysm syndrome TGFBR1 9q22, TGFBR2 3p22
P155-EDS The Ehlers-Danlos syndrome type III (EDS III) COL3A1 2q31, TNXB 6p21.3.
P191-Alport-mix1 Alport syndrome, Hereditary Nephritis COL4A5 Xq22
P192-Alport-mix2 Alport syndrome, Hereditary Nephritis COL4A5 Xq22
P214-COL2A1 Skeletal disorders, Achondrogenesis, Chondrodysplasia, Early onset familial Osteoarthritis, SED congenital, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome, Spondyloepimetaphyseal COL2A1 12q13.11-q13.2
P215-EXT Multiple Osteochondromas EXT1 8q24, EXT2 11p11
P223-PHEX Hypophosphatemia, x-linked PHEX Xp22.2, FGF23 12p13
P228-TRPS1-EXT1 Trichorhinophalangeal syndrome type I (TRPS1), Langer-Giedion syndrome (LGS) TRPS1, EXT1, 8q24
P271-COL1A1 Osteogenesis imperfecta (OI) COL1A1 17q21.3
P272-COL1A2 Osteogenesis imperfecta (OI) COL1A2 7q21.3
P285-LRP5 Osteoporosis-pseudoglioma syndrome (OPPS) LRP5 11q13.4, DKK1 10q11.2
P289-LMX1B Nail patella syndrome (NPS) LMX1B 9q33
P331-COL5A1 MIX-1 Ehlers-Danlos Syndrome COL5A1 9q34.3
P332-COL5A1 MIX-2improved Ehlers-Danlos Syndrome COL5A1 9q34.3
P359-PLOD1 Ehlers-Danlos syndrome type VI; Kyphoscoliotic Form 1p36.22
P381-COL11A1 mix 1 Marshall, type II Stickler syndromes COL11A1
P382-COL11A1 mix 2 Marshall, type II Stickler syndromes COL11A1
P415-COL7A1-KRT5 Epidermolysis Bullosa Hereditaria 3p21.31 COL7A1, 12q13.13 KRT5
P416-KRT14-LAMA3-LAMB3-LAMC2 Epidermolysis Bullosa Hereditaria 17q21.2 KRT14; 18q11.2 LAMA3; 1q32.2 LAMB3; 1q25.3 LAMC2
P435-FLNB Atelosteogenesis, Boomerang dysplasia, AD Larsen Syndrome 3p14.3 FLNB
P439-COL4A3 Alport Syndrome 02q36.3
P444-COL4A4 Alport Syndrome 2q36.3
P452-PLS3 Osteogenesis imperfecta Xq23
P456-EVC EVC2 Ellis-van Creveld syndrome 4p16.2
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