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SALSA MLPA Reagent Kits for Solid Tumors

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SALSA MLPA Reagent Kits for Solid Tumors
MLPA (Multiplex Ligation-dependant Probe Amplification (MLPA) is a multiplex PCR method that enables the copy number variation (CV) detection of up to 60 DNA sequences in a single reaction. 96 DNA samples can be handled simultaneously with results being available within 24 hours.
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Features & Benefits

  • Detection of Solid Tumors
  • Simultaneous Detection of copy number, methylation and known point mutation
  • Low Input - Requires only 50 ng of DNA
  • Reproducible - High concordance with other techniques
  • Time Efficient - Results available within 24 hrs.
  • High Throughput - Can detect up to 60 sequences in 96  DNA samples, in one reactions
  • Cost Effective

Product Details

MLPA® is the gold standard for DNA copy number quantification and is used to study both hereditary disorders and tumours. MLPA can also be applied to investigate the methylation status of DNA sequences. MRC-Holland, the inventor and manufacturer of MLPA, offers 400 different MLPA panels. MLPA (Multiplex Ligation-dependant Probe Amplification (MLPA) is a multiplex PCR method that enables the copy number variation (CV) detection of up to 60 DNA sequences in a single reaction. 96 DNA samples can be handled simultaneously with results being available within 24 hours.


Components provided per SALSA MLPA Reagent Kit  



  • SALSA MLPA Buffer (yellow cap)

  • SALSA Ligase-65 (green cap)

  • Ligase Buffer A (transparent cap)

  • Ligase Buffer B (white cap)

  • SALSA PCR Primer Mix (brown cap)

  • SALSA Polymerase (orange cap) 


MLPA has the following solutions for Solid Tumors:


























































































































































































































































PRODUCT NAME APPLICATION REGION
ME001-Tumour suppressor mix 1basic research Tumour suppressor genes Various
ME002-Tumour suppressor mix 2basic research Tumour suppressor genes Various
ME011-MMR Mismatch repair genes (MMR) MLH1, MSH2, MSH6, MLH3, MSH3, PMS2, MGMT
ME012-MGMT-IDH1-IDH2 Gliomas MGMT, IDH1, IDH2
ME024-9p21 Tumours, Cutaneous melanoma 9p21, CDKN2A, CDKNA2B
ME042-CIMPbasic research CpG Island Methylator Phenotype Various
P004-ERBB2 Cancer ERBB2 (HER2-NEU) 17q21.1
P014-Chromosome 8 Tumour research Chromosome 8
P027-Uveal melanoma Uveal melanoma 1p, chr.3, 6p, 8q
P047-RB1 Retinoblastoma (RB) RB1 13q14
P056-TP53 Li-Fraumeni syndrome (LFS) and tumour analysis TP53 17p13.1
P063-FHIT-WWOX Diverse tumour types, epileptic encephalopathy FHIT (3p14.2) and WWOX (16q23.1)
P078-Breast tumour Breast tumour various
P083-CDH1 Hereditary diffuse gastric cancer CDH1 at 16q22.1
P088-Oligodendroglioma 1p-19q Oligodendroglioma 1p, 19q, IDH1/2, CDKN2A/2B
P105-Glioma-2 Glioma, malignant EGFR, TP53, PTEN + various other
P146-CRC GAINbasic research Colon cancer Various
P157-20q region Colon cancer, breast cancer and various others 20q (BCL2L1, MYBL2, ZNF217, DNMT3B, AURKA, GNAS)
P175-Tumour Gainbasic research Tumour Gain various
P181-Centromere mix 1 Centromeric screening Centromeres
P182-Centromere mix 2 Centromeric testing Centromeres
P215-EXT Multiple Osteochondromas EXT1 8q24, EXT2 11p11
P225-PTEN Cowden syndrome and tumour analysis PTEN 10q23
P251-NB mix 1 Neuroblastoma 1p36, 3p22/3p21.3 and 11q23
P252-NB mix 2 Neuroblastoma 2p24.1/MYCN, 2q33, 17p13/TP53, 17q
P253-NB mix 3 Neuroblastoma Chromosomes 4, 7, 9, 12, 14
P258-SMARCB1 Rhabdoid tumors SMARCB1 22q11.2
P294-Tumour Lossbasic research Tumour Loss various
P298-BRAF-HRAS-KRAS-NRASbasic research Various tumour types BRAF, HRAS, KRAS, NRAS
P301-Medulloblastoma mix 1 Medulloblastoma Chr. 6, Chr. 14, Chr. 16, Chr. 17.
P302-Medulloblastoma mix 2 Medulloblastoma Chr. 2, Chr. 3, Chr. 7, Chr. 9
P303-Medulloblastoma mix 3 Medulloblastoma Chr. 1, Chr. 4, Chr. 5, Chr. 8, Chr. 10, Chr. 20
P308-MET Papillary renal carcinoma, various cancers MET 7q31, PTEN 10q23.31, LRRK2 12q12
P315-EGFR Tumours, solid EGFR 7p11
P323-CDK4-HMGA2-MDM2 Sarcoma and other tumour types chromosome 12, MDM2, CDK4 and HMGA2 genes
P370-BRAF-IDH1-IDH2basic research Gliomas BRAF, IDH1, IDH2, FGFR1, MYB, MYBL1, CDKN2A
P376-BRCA1ness BRCA1-like breast cancer profile various
P378-MUTYH Colon cancer, stomach cancer (hereditary) 1p34 and 15q13
P380-Wilms' tumour Wilms' tumour various
P413-CRC LOSSbasic research Colorectal adenoma/carcinoma 8p, 15q, 17p, 18q
P417-BAP1 Melanocytic tumours, mesothelioma BAP1, 3p21.1
P419-CDKN2A/2B-CDK4 Familial melanoma CDKN2A, CDKN2B, CDK4, MITF E318K
P429-SDHA-MAX Paraganglioma and pheochromocytoma 5p15.3 and 14q23.3
P433-ARID1A-ARID1B Neuroblastoma, ovarian cancer + various other tumour types + mental retardation ARID1A, ARID1B
P448-PARD3 PARD3 10p11.21-p11.22
P455-LZTR1 Schwannomatosis 22q11.21
P458-Gastric cancer Gastric cancer various
P466-CDC73 HPT jaw tumour syndrome, famial hyperparathyroidism, parathyroid carcinoma CDC73
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