Clonality based testing is done for the identification of clonal B-cell populations highly suggestive of B-cell malignancies. It can help in the following ways:
- Lineage determination of leukemias and lymphomas
- Monitoring and evaluation of disease recurrence
- Detection and assessment of residual disease
- Evaluation of new research and methods in malignancy studies
Some examples of ABI Fluorescence Detection Assays from Invivoscribe are: IGH Gene clonality Assay, IGK Gene Clonality Assay, TCRB+TCRG T-cell Clonality Assay
ABI Fluorescence Detection is done using ABI 310, 3100 & 3130 instruments
Due to instrument to instrument variation in the performance of the ABI 3500 platform, the amount of formamide, sample, and size standard listed in the protocol is intended to be a starting point. The protocol may need to be optimized for specific ABI 3500 Platforms.