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3i Lattice LightSheet
Lattice LightSheet uses ultra-thin sheets of light to image 3D cellular dynamics for hundreds of volumes at dozens of frames per second at diffraction-limited resolution and super-resolution. Invented by Nobel Laureate Dr. Eric Betzig of the Howard Hughes Medical Institute Janelia Research Campus, this microscope has been applied to biological systems spanning four orders of magnitude in space and time. An extremely sensitive primary objective coupled with a custom-designed illumination system allows optical sectioning using extremely low light doses for imaging with unprecedented duration. 3D experiments previously limited by phototoxicity in just seconds or minutes can now be continued safely for hours or days. The combination of high spatiotemporal resolution, speed and sensitivity make the Lattice LightSheet the ultimate tool in a new era for living cell microscopy.
Early successful applications of this instrument include the diffusion of single transcription factors in a spheroid of stem cells, 3D dynamic instability of microtubules during mitosis, formation of the immunological synapse in T cell/antigen presenting cell (APC) interactions, neutrophil motility in a 3D matrix, and embryogenesis in C elegans and Drosophila melanogaster.
About
3i Imaging Instruments in India
3i was established in 1995 by a group of young and dynamic scientists working in the fields of cell biology, immunology, neuroscience, and computer science. At 3i, they aim to provide advanced multidimensional microscopy platforms, with its modular design and user-friendly technology, that meet the evolving needs of the biological research community.
3i (Intelligent Imaging Innovations) designers and manufacturers provide cutting-edge live cell and intravital microscopy imaging platforms that are driven by 64-bit SlideBook software. DSS is a key distribution partner for their representation in India.
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PGIMER, Chandigarh
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MD, Spermprocessor Pvt Ltd
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Dr. Sreejesh S, Associate Professor, Dept of Hematology
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RMLIMS, Lucknow
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Assistant Professor, PGIMER, Chandigarh
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Mr. Krishnani Professor, SGPGI, Lucknow
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