Methylation-Specific MLPA Assays

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Methylation-Specific (MS)-MLPA® is a variant of the MLPA technique, the gold standard in copy number determination. With MS-MLPA, it is possible to semi-quantitatively profile methylation for multiple targets simultaneously.

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Features & Benefits

MS-MLPA® is frequently used to detect imprinting region methylation, like the Prader-Willi/Angelman (ME028 PWS/AS) and Beckwith-Wiedemann/Russell-Silver (ME030 BWS/RSS) syndromes critical regions.
The most important difference between MLPA and MS-MLPA is that in MS-MLPA the reaction is split into an undigested and a digested reaction after overnight hybridisation.

Product Details

Methylation-Specific (MS)-MLPA® is a variant of the MLPA technique, the gold standard in copy number determination. By combining the MLPA technique with the use of the methylation-sensitive endonuclease HhaI, it is possible to detect both DNA copy number and methylation status without bisulfite treatment. With MS-MLPA, semiquantitative methylation profiling of multiple targets is accomplished simultaneously and with ease thereby aiding in imprinting disorders