Entrogen's Real Time PCR Kits for Colorectal Cancer are available as following three categories :
|S.No.||Product Name||Catalog Number||Platform|
|1||KRAS Mutation Analysis Kit||KRAS-RT50||For Real-Time PCR|
|2||KRAS/BRAF Mutation Analysis Kit||KRBR-RT50||For Real-Time PCR|
|3||RAS Mutation Analysis Kit||RAS-RT50||For Real-Time PCR|
|4||AKT E17K Mutation Analysis Kit||AKTE17-RT50||For Real-Time PCR|
|5||PIK3CA Mutation Analysis Kit||PI3K-RT48||For Real-Time PCR|
|6||NRAS Mutation Analysis Kit||NRAS-RT50||For Real-Time PCR|
|7||Colorectal Cancer Mutation Detection Panel||CRC-RT48||For Real Time PCR|
The above kits are polymerase chain reaction (PCR)-based assays that use allele-specific primers in a multiplex reaction to identify the presence of KRAS, NRAS, BRAF, PIK3CA and/or AKT1 mutations. The assays work by amplifying mutant-specific sequences in samples that contain a mixture of mutant and wild-type DNA and rely on fluorescent probes for detection. Each reaction contains primer sets and probes for detection of the mutations, as well as an endogenous control gene. The testing procedure involves three (3) simple steps:
1. Isolation of DNA from tumor biopsies, paraffin-embedded sections (FFPE), fresh frozen tumors, or plasma (for cell free kit).
2. Amplification using the provided reagents in the kit.
3. Data analysis and interpretation using the real-time PCR software or provided analysis worksheet
All kits require a real-time PCR instrument capable of detecting FAM and VIC fluorescent probes, while the Colorectal Cancer Mutation Detection Panel requires an instrument able to additionally detect ROX and CY5 fluorescent probes. All reagents required for PCR amplification/detection, as well as validated reaction controls are included. Columns and reagents for DNA isolation are not included.