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diSPIM

diSPIM
Dual-view Inverted Selective Plane Illumination (diSPIM) is a light sheet method offering low phototoxicity in 3D imaging making it possible to image living small organisms over extended periods of time.
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Dual-view Inverted Selective Plane Illumination (diSPIM) is a light sheet method offering low phototoxicity in 3D imaging making it possible to image living small organisms over extended periods of time. It is a flexible light sheet system with an open specimen geometry not requiring special slides or capillaries. diSPIM images a specimen using two perpendicular light sheets from two equivalent optical paths and objectives which are each used sequentially for both illumination and imaging. The resulting 3D datasets have isotropic resolution in X, Y and Z with only two views, resulting in fast image capture with low light dose for extended specimen viability.

Two matching water dipping objectives can have an NA of up to 0.8 with a working distance allowing efficient orthogonal mounting. Each objective is used for excitation and emission collection via identical high-speed high-resolution sCMOS cameras. A third objective is located below the specimen chamber for locating the sample. Photomanipulation can be added via the third objective, such as the Ablate photoablation system.
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