Multiphoton excitation microscopy is more related to non-linear, multiphoton, or two-photon laser scanning microscopy. It is an alternative to confocal and deconvolution microscopy that renders distinct support for three-dimensional imaging. Indistinct, two-photon excitation surpasses the imaging of living cells, especially within the tissues such as brain slices, embryos, whole organs, and also in entire animals. The adequate sensitivity of fluorescence microscopy, particularly with thick specimens, is generally bounded by out-of-focus flare. This condition is greatly reduced in a confocal microscope, by the use of a confocal pinhole to decline out-of-focus background fluorescence and give thin (less than 1 micrometre), unblurred optical slices. Alternatively, deconvolution microscopy uses a traditional microscope to digitally reproduce an image by utilising the measured point spread function of the optical system.
With the Olympus FVMPE-RS, countless possibilities for deep tissue observation are finally realized. The system delivers unmatched high-speed imaging, essential for capturing the dynamic in vivo response, with fine laser control pinpointing the precise site for optimum excitation efficiency - even deep within the sample.ADD TO SHORTLIST