Cell culture is an in-vitro process through which cells are separated from animal or plant tissue. Their subsequent growth is maintained and controlled by artificial physical and chemical conditions which usually mimics in-vivo condition of that cell type.
Nowadays, in-vitro cell culture has become a common practice in many laboratories for large number of applications.
Cell culture provides an ideal model system for understanding the normal physiology and biochemistry of cells. It also helps to study the effect of drugs and toxic compounds on cells, mutagenesis and carcinogenesis.
It plays a major role in drug screening and development process and in large scale manufacturing of biological compounds (e.g., vaccines, therapeutic proteins).
Reproducibility of results is a major advantage of using cell culture techniques.
Primary cell culture: The cells are first isolated from tissue and then proliferated under the controlled and feasible conditions.
Cell Line: A cell line is a permanently established cell culture that will proliferate indefinitely in suitable fresh medium and space.
Cell Strain: A cell line which is positively selected from cell culture is called a Cell Strain.
Finite Cell Line: Cell culture which divides only a limited number of times before losing their ability to proliferate, are known as Finite Cell Line.
Continuous Cell Line: A finite cell line which undergoes a chemical or virally induced transformation, acquires the ability to divide indefinitely becomes Continuous Cell Line.
Cells in culture can be divided into three major categories based on their morphology
- Fibroblast-Like cells
- Epithelial-Like cells
- Lymphoblast-Like cells
DSS provides complete Cell Culture solutions:
- Euroclone media to culture different type of cells.
- Cell counter R1 to count cultured cells.
- Olympus CKX53 Inverted microscope to visualize live cells, image capture and fluorescence observation.
The Olympus CKX53 microscope delivers excellent performance, with improved image quality and ergonomics, as well as a comfortable workflow for a variety of cell culture needs such as live cell observation, cell sampling and handling, fluorescence observation, and image capture.ADD TO SHORTLIST