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Abbott RealTime HIV-1 Assay

Abbott RealTime HIV-1 Assay
The Abbott RealTime HIV-1 assay is an in vitro reverse transcription-polymerase chain reaction (RT-PCR) assay for the quantitation of Human Immunodeficiency Virus type 1 (HIV-1) on the automated m2000 System in human plasma from HIV-1 infected individuals over the range of 40 to 10,000,000 copies/mL. The Abbott RealTime HIV-1 assay is intended for use in conjunction with clinical presentation and other laboratory markers for disease prognosis and for use as an aid in assessing viral response to antiretroviral treatment as measured by changes in plasma HIV-1 RNA levels.
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Features & Benefits

    The Abbott RealTime HIV-1 assay is designed to detect and quantify all HIV-1 Group M subtypes A–H, Group O, and Group N and provide sensitivity, specificity, and dynamic range to meet your laboratory needs.

  • Enhanced precision – Provides excellent performance measuring low level viremia
  • Accurate monitoring – Confidence to reliably detect changes to patient’s response status
  • Unique assay design – Partially double stranded probe, highly conserved target region and specific temperature profile ensure reliable detection of all groups and subtypes

Product Details

The Abbott RealTime HIV-1 assay is an in vitro reverse transcription-polymerase chain reaction (RT-PCR) assay for the quantitation of Human Immunodeficiency Virus type 1 (HIV-1) on the automated m2000 System in human plasma from HIV-1 infected individuals over the range of 40 to 10,000,000 copies/mL. The Abbott RealTime HIV-1 assay is intended for use in conjunction with clinical presentation and other laboratory markers for disease prognosis and for use as an aid in assessing viral response to antiretroviral treatment as measured by changes in plasma HIV-1 RNA levels.


HIV-1 diversity can be attributed to:



  • Error-prone reverse transcriptase enzyme

  • Recombination of subtypes

  • Cross-species transmission


Accurate quantification is dependent upon a combination of:



  • Primer design

  • Probe design

  • Cycling conditions


The Abbott RealTime HIV-1 assay is designed to detect and quantify all HIV-1 Group M subtypes A–H, Group O, and Group N and provide sensitivity, specificity, and dynamic range to meet your laboratory needs.



This assay is not intended to be used as a donor screening test for HIV-1 or as a diagnostic test to confirm the presence of HIV-1 infection.

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